| 质粒名称: | pCMV-MCP-HA-SFV nsP3 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002869 |
| 产品用途: | Mammalian expression of MCP-HA-SFV nsP3 |
| 存储实验室: | Peiguo YANG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-C1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SFV nsP3 |
| 物种来源: | Alphavirus |
| 密码子优化: | 人源表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Alphaviral non-structural protein-host RBP co-condensation as a mechanism to sustain virus replication
Molecular Cell, Volume 86, Issue 9, 2026
Yi Liu, Peiguo Yang
|
pCMV-MCP-HA-SFV nsP3
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCMV-MCP-HA-SFV nsP3载体质粒基本信息
pCMV-MCP-HA-SFV nsP3质粒图谱载体图谱和pCMV-MCP-HA-SFV nsP3载体序列质粒序列多克隆位点信息
pCMV-MCP-HA-SFV nsP3质粒载体简介
pCMV-MCP-HA-SFV nsP3质粒序列载体序列
LOCUS pCMV-MCP-HA-SFV nsP3 5832 bp DNA circular SYN 16-JUL-2026
DEFINITION Localization: C1 Cloning Vector, Excitation: 488, Emission: 507.
ACCESSION .
VERSION .
KEYWORDS pCMV-MCP-HA-SFV nsP3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5832)
TITLE Michael Davidson Empty Backbones
REFERENCE 2 (bases 1 to 5832)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 7, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5832
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 142..445
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 446..649
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 600..620
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind complement(672..708)
/label=引物 6
primer_bind 679..717
/label=引物 1
primer_bind complement(682..693)
/label=p61-1052
regulatory 688..693
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 694..1080
/codon_start=1
/label=MCP
/translation="MLAVKMASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRS
QAYKVTCSVRQSSAQNRKYTIKVEVPKGAWRSYLNMELTIPIFATNSDCELIVKAMQGL
LKDGNPIPSAIAANSGIYADPPVAT"
primer_bind complement(1069..1107)
/label=p61-1052
primer_bind 1093..1141
/label=p61-1051
CDS 1093..1119
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 1141..2589
/codon_start=1
/label=SFV nsP3 (human codon)
/translation="APSYRVKRADIATCTEAAVVNAANARGTVGDGVCRAVAKKWPSAF
KGAATPVGTIKTVMCGSYPVIHAVAPNFSATTEAEGDRELAAVYRAVAAEVNRLSLSSV
AIPLLSTGVFSGGRDRLQQSLNHLFTAMDATDADVTIYCRDKSWEKKIQEAIDMRTAVE
LLNDDVELTTDLVRVHPDSSLVGRKGYSTTDGSLYSYFEGTKFNQAAIDMAEILTLWPR
LQEANEQICLYALGETMDNIRSKCPVNDSDSSTPPRTVPCLCRYAMTAERIARLRSHQV
KSMVVCSSFPLPKYHVDGVQKVKCEKGLLFDPTVPSVVSPRKYAASTTDHSDRSLRGFD
LDWTTDSSSTASDTMSLPSLQSCDIDSIYEPMAPIVVTADVHPEPAGIADLAADVHPEP
ADHVDLENPIPPPRPKRAAYLASRAAERPVPAPRKPTPAPRTAFRNKLPLTFGDFDEHE
VDALASGITFGDFDDVLRLGRAGA"
primer_bind complement(2566..2604)
/label=p61-1020
primer_bind 2575..2613
/label=p61-1021
polyA_signal 2701..2822
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2738..2757)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2792..2811
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2829..3284)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2966..2987)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3178..3197
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3311..3415
/gene="bla"
/label=AmpR promoter
promoter 3417..3774
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3440..3460)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3625..3760
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3687..3706
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(3710..3721)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3809..4603
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3863..3882)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4473..4492
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4791..4810)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4835..4882
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5211..5799
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5700..5719
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
61 aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt cattagttca
121 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
181 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
241 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
301 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
361 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
421 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
481 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
541 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
601 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
661 ccgtcagatc cgctagcgct accggtcgcc accatgctag ccgttaaaat ggcttctaac
721 tttactcagt tcgttctcgt cgacaatggc ggaactggcg acgtgactgt cgccccaagc
781 aacttcgcta acgggatcgc tgaatggatc agctctaact cgcgttcaca ggcttacaaa
841 gtaacctgta gcgttcgtca gagctctgcg cagaatcgca aatacaccat caaagtcgag
901 gtgcctaaag gcgcctggcg ttcgtactta aatatggaac taaccattcc aattttcgcc
961 acgaattccg actgcgagct tattgttaag gcaatgcaag gtctcctaaa agatggaaac
1021 ccgattccct cagcaatcgc agcaaactcc ggcatctacg cggatccacc ggtcgccacc
1081 ccggtcgcca cctatccata tgacgttcca gattacgctt actcagatct cgagctcaag
1141 gctcctagtt atcgggtgaa gcgcgcggac attgctactt gtaccgaagc cgccgtagtc
1201 aacgccgcca atgctcgcgg caccgtgggc gatggagtct gccgggccgt cgcgaaaaag
1261 tggccgagtg cgttcaaagg cgcggcaacc ccagttggga caattaagac cgtcatgtgt
1321 ggcagctatc cggtcatcca cgccgtcgcc cctaacttct cagcaacaac cgaggctgaa
1381 ggggaccggg aactcgccgc ggtatataga gcggtggcag ccgaggtaaa cagattgtcc
1441 ttgagttccg ttgcaattcc ccttcttagc acaggtgtgt ttagcggtgg gcgcgacaga
1501 ctgcagcagt ccctgaacca tctctttact gccatggatg cgacggatgc ggatgtcacc
1561 atctattgcc gcgataagag ttgggagaag aagattcaag aggccattga tatgaggacc
1621 gcagtagagc tgctgaatga cgacgtagag cttacgacag acctggtcag agttcatccc
1681 gacagttccc tcgttggaag aaagggttac agcacgactg acggatcctt gtactcatac
1741 tttgaaggta ctaagttcaa ccaagctgcg attgatatgg ccgaaatttt gacgttgtgg
1801 ccacgcctcc aagaggcgaa cgagcaaata tgtctgtatg ctctcgggga gactatggac
1861 aacattcgaa gtaaatgccc agtcaacgat tcagattcta gcactccccc taggactgtc
1921 ccctgcttgt gtaggtacgc catgacggcc gagcgaatag ctcggcttcg ctctcatcaa
1981 gtcaaatcaa tggtggtatg ttcaagtttc ccccttccga aataccatgt agacggggtc
2041 cagaaagtca agtgcgaaaa aggtcttttg ttcgatccta ctgtaccctc tgtagtctct
2101 ccgaggaagt atgccgctag taccacagat cactccgaca ggagtttgcg ggggttcgac
2161 cttgattgga ccactgactc ttcctcaacg gctagtgata cgatgtcatt gccaagcctg
2221 caatcatgtg acattgacag tatctatgag ccgatggctc ccattgtggt cacggctgat
2281 gtacatccag aacccgctgg aatcgcagac cttgcggctg acgttcaccc cgaaccagca
2341 gatcacgttg acctggagaa tccgattcct cctccgaggc ctaagagagc cgcgtacctg
2401 gcatcacggg cggctgaacg gcccgtccct gcgccaagga aacccactcc cgccccacgc
2461 actgcattta gaaataagct ccccctgact ttcggagatt tcgatgaaca tgaagtagac
2521 gcgctcgcta gtgggataac ttttggagat tttgatgacg tcctccgact gggaagagct
2581 ggggcttagt aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta
2641 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
2701 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
2761 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
2821 taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
2881 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
2941 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
3001 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
3061 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
3121 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
3181 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
3241 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
3301 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
3361 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga
3421 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
3481 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
3541 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
3601 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
3661 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
3721 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga
3781 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
3841 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
3901 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
3961 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
4021 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
4081 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
4141 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
4201 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
4261 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
4321 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
4381 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
4441 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
4501 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
4561 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
4621 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
4681 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
4741 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg
4801 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
4861 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
4921 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
4981 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
5041 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt
5101 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
5161 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
5221 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
5281 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
5341 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
5401 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
5461 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
5521 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
5581 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
5641 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
5701 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
5761 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
5821 ttacggttcc tg
//
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